Journal: International Journal of Genomics
Article Title: Comprehensive Analysis to Identify LINC00511–hsa-miR-625-5p–SEMA6A Pathway Fuels Progression of Skin Cutaneous Melanoma
doi: 10.1155/2023/6422941
Figure Lengend Snippet: Representative immunohistochemistry images of SEMA6A in SKCM tissues and normal skin tissues (HPA) (a) SEMA6A expression level, ∗ represents p < 0.05 (b). The results of quantitative reverse transcription PCR (RT-qPCR) of SEMA6A expression level in SKCM cell lines (c) ∗∗∗, ∗∗, and ∗ represent significant differences compared with human primary normal skin melanocytes ( p < 0.001, p < 0.01, and p < 0.05), respectively.
Article Snippet: Based on the manufacturer's instructions, the study involved the extraction of total RNAs from both melanoma cell lines and human primary normal skin melanocyte cell lines using TRIzol (Takara Biomedical Technology (Beijing) Co., Ltd., Beijing, China).
Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR